Sterile Media Filtration & Cultureware

Stericup® Sterile Vacuum Filtration Systems are known to research scientists worldwide for reliable, high-performance membranes and optimum sterile filtration of laboratory reagents, buffers, and media with capacity fom 150ml to 1000ml.

Whether your goal is a green lab certification, or just a smaller footprint, both the Stericup® E and Steritop® E Filtration Systems are designed to ensure trouble-free cell culture, while diminishing environmental impact in multiple ways.

Millex® Syringe Filters are ideal for sterilizing 1 mL – 200 mL of cell culture reagents, such as cell culture media, antibiotics, supplements, sera, viruses for infection, and other biological solutions.

DMEM (Dulbecco's Modified Eagle Medium) is the most broadly suitable medium for many adherent cell phenotypes among defined media for cell and tissue culture.

RPMI-1640 Media was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.

The ability to successfully freeze cells and resuscitate them from liquid nitrogen storage is one of the most important aspects of cell culture research. Preventing ice crystal formation in particular is paramount to maintaining cell viability during cryopreservation.

With more than 35 years of experience in the management and production of Sera for use in cell culture, Sigma Aldrich offers many varieties of bovine serum (cow), chicken, caprine (goat), equine (horse), human, ovine (sheep), porcine (pig) and rabbit sera produced and tested for use in cell culture applications.

Reagents & Supplements

Sigma Aldrich offers a wide variety of supplements, reagents and growth factors, including:

Cell Supplements: Cell Culture and Insect Cell Culture tested supplements undergo additional testing within a cell culture system. Product ranges include albumins and transport proteins; amino acids and vitamins; and antibiotics.

Cell Dissociation Reagents: Sigma Aldrich offers many solutions for cell dissociations from Trypisin EDTA, StableCell™ Trypsin Solution (Stable at 2-8 °C), Collagenase, Accumax™, Accutase® and other Non-Enzymatic Reagents for Gentle Cell Dissociation.

Growth Factors & Cytokines: Growth factors are the proteins that bind to receptors on the cell surface of non-hematopoietic cells and result in proliferation or differentiation of the affected cells. Some of the common offerings include: Epidermal Growth Factors (EGF) - (33); Fibroblast Growth Factors (FGF) - (45); HumanKine Growth Factors & Cytokines - (33), among others.

The Scepter™ cell counter provides true automation without the error that accompanies vision-based systems. With its microfabricated, precision-engineered sensor, the Scepter™ cell counter does all the work and delivers accurate and reliable cell counts in less than 30 seconds.

Plates & Membrane-based Cultureware

MultiScreen® Filter Plates are available in a broad selection of stock configurations. Plates with 96- and 384-well capacity are manufactured using a diverse range of housing materials to ensure compatibility with varied media formulations.

Millicell® Membrane-based Plates & Inserts are designed to support cell growth and differentiation of epithelial and endothelial cell lines, including CaCo-2. Optically-suitable organotypic inserts are designed for convenient access and provide sustained viability. The Millicell device is available with a 0.4 µm PCF membrane, along with a variety of PET membrane pore sizes.

Gulf Scientific Corporation (GSC) is a leading provider of complete laboratory solutions in the fields of analytical, life and material sciences. Founded in 1990, GSC is headquartered in Dubai, United Arab Emirates, with regional offices and supply chain distribution centers across the Middle East.