Sterile Media Filtration & Cultureware
Stericup® Sterile Vacuum Filtration Systems are known to research scientists worldwide for reliable, high-performance membranes and optimum sterile filtration of laboratory reagents, buffers, and media with capacity fom 150ml to 1000ml.
Whether your goal is a green lab certification, or just a smaller footprint, both the Stericup® E and Steritop® E Filtration Systems are designed to ensure trouble-free cell culture, while diminishing environmental impact in multiple ways.
Millex® Syringe Filters are ideal for sterilizing 1 mL – 200 mL of cell culture reagents, such as cell culture media, antibiotics, supplements, sera, viruses for infection, and other biological solutions.
DMEM (Dulbecco’s Modified Eagle Medium) is the most broadly suitable medium for many adherent cell phenotypes among defined media for cell and tissue culture.
RPMI-1640 Media was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.
The ability to successfully freeze cells and resuscitate them from liquid nitrogen storage is one of the most important aspects of cell culture research. Preventing ice crystal formation in particular is paramount to maintaining cell viability during cryopreservation.
With more than 35 years of experience in the management and production of Sera for use in cell culture, Sigma Aldrich offers many varieties of bovine serum (cow), chicken, caprine (goat), equine (horse), human, ovine (sheep), porcine (pig) and rabbit sera produced and tested for use in cell culture applications.
Reagents & Supplements
Sigma Aldrich offers a wide variety of supplements, reagents and growth factors, including:
• Cell Supplements: Cell Culture and Insect Cell Culture tested supplements undergo additional testing within a cell culture system. Product ranges include albumins and transport proteins; amino acids and vitamins; and antibiotics.
• Cell Dissociation Reagents: Sigma Aldrich offers many solutions for cell dissociations from Trypisin EDTA, StableCell™ Trypsin Solution (Stable at 2-8 °C), Collagenase, Accumax™, Accutase® and other Non-Enzymatic Reagents for Gentle Cell Dissociation.
• Growth Factors & Cytokines: Growth factors are the proteins that bind to receptors on the cell surface of non-hematopoietic cells and result in proliferation or differentiation of the affected cells. Some of the common offerings include: Epidermal Growth Factors (EGF) – (33); Fibroblast Growth Factors (FGF) – (45); HumanKine Growth Factors & Cytokines – (33), among others.
Plates & Membrane-based Cultureware
MultiScreen® Filter Plates are available in a broad selection of stock configurations. Plates with 96- and 384-well capacity are manufactured using a diverse range of housing materials to ensure compatibility with varied media formulations.
Millicell® Membrane-based Plates & Inserts are designed to support cell growth and differentiation of epithelial and endothelial cell lines, including CaCo-2. Optically-suitable organotypic inserts are designed for convenient access and provide sustained viability. The Millicell device is available with a 0.4 µm PCF membrane, along with a variety of PET membrane pore sizes.
Introducing the Scepter™ 3.0
The Scepter™ 3.0 cell counter provides the unparalleled precision of Coulter impedance counting in a convenient, handheld format. The latest version of the popular Scepter cell counter has been updated with features that make it easier to use, as well as more convenient and accurate than ever before. The Scepter 3.0 cell counter provides true automation without the risk of error that accompanies vision-based systems.
Portable Coulter Counter Precision
See How it Works
Besides being the only handheld automated cell counter, the Scepter™ 3.0 counter brings Coulter-based accuracy that visual counters can’t provide. Find out how it works, and learn more about the performance data.
The Scepter™ cell counter provides true automation without the error that accompanies vision-based systems. With its microfabricated, precision-engineered sensor, the Scepter™ cell counter does all the work and delivers accurate and reliable cell counts in less than 30 seconds.